Interactions of monomolecular films of retinal at alkaline pH.

Interactions between retinal and phospholipids in monomolecular films at acid pH.

The surface properties of mixed monomolecular films of retinal and phospholipids (p. lipids) are measured as a function of mole fraction at a nitrogen-water interface. An acid pH of 6.0 is maintained in the aqueous phase. Before irradiation the surface potential deltaV for 9-cis retinal, 11-cis retinal, phosphatidyl serine (PS) and phosphatidyl ethanolamine (PE), at pi=12 dyn/cm, are 490 mV, 64...

Interactions of neomycin with monomolecular films of polyphosphoinositides and other lipids.

The interactions of calcium and the aminoglycosidic antibiotic, neomycin, with various lipids were investigated in monomolecular films. Lipids were spread over a subphase of 0.05 M N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, pH 7.0, and NaCl to give an ionic strength of 0.2. Measurements of surface pressure (pi) were taken with a Wilhelmy balance. In the absence of Ca2+, 1 muM--1 mM ne...

Retinals in a Model Membrane System

Monomolecular films of 9-cis, 11-c/5, 13-cis and all-trans retinal were formed at an air-water interface. Area/molecule and surface potential were measured before, during and after illumina­ tion. The initial quantum yield of the photoisomerization o f 9-cis retinal was 0.25. Irradiation of a retinal monolayer resulted in 30 to 60 m Volt changes in surface potential. Complexation of retinals wi...

A new method for investigation of lipid assemblies with a lipoid pH indicator in monomolecular films.

Further biochemical characterization of human pancreatic lipase-related protein 2 expressed in yeast cells.

Recombinant human pancreatic lipase-related protein 2 (rHPLRP2) was produced in the protease A-deficient yeast Pichia pastoris. A major protein with a molecular mass of 50 kDa was purified from the culture medium using SP-Sepharose and Mono Q chromatography. The protein was found to be highly sensitive to the proteolytic cleavage of a peptide bond in the lid domain. The proteolytic cleavage pro...